Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NFkappaB

2014 | Retrovirology

Program(s): Laboratory |

Abstract:

BACKGROUND: Molecular latency allows HIV-1 to persist in resting memory CD4+ T-cells as transcriptionally silent provirus integrated into host chromosomal DNA. Multiple transcriptional regulatory mechanisms for HIV-1 latency have been described in the context of progressive epigenetic silencing and maintenance. However, our understanding of the determinants critical for the establishment of latency in newly infected cells is limited.

RESULTS: In this study, we used a recently described, doubly fluorescent HIV-1 latency model to dissect the role of proviral integration sites and cellular activation state on direct non-productive infections at the single cell level. Proviral integration site mapping of infected Jurkat T-cells revealed that productively and non-productively infected cells are indistinguishable in terms of genomic landmarks, surrounding epigenetic landscapes, and proviral orientation relative to host genes. However, direct non-productive infections were inversely correlated with both cellular activation state and NFkappaB activity. Furthermore, modulating NFkappaB with either small molecules or by conditional overexpression of NFkappaB subunits was sufficient to alter the propensity of HIV-1 to directly enter a non-productive latent state in newly infected cells. Importantly, this modulatory effect was limited to a short time window post-infection.

CONCLUSIONS: Taken together, our data suggest that cellular activation state and NFkappaB activity during the time of infection, but not the site of proviral integration, are important regulators of direct HIV-1 non-productive infections.

BC Centre for Excellence in HIV/AIDS

Search form

Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NFkappaB

Search form